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Ortsgerichtete Rekombination in Chlamydomonas reinhardtii am Beispiel des Cre/lox-Systems

URN to cite this document: urn:nbn:de:bvb:355-opus-8808

Mägdefrau, Marion (2008) Ortsgerichtete Rekombination in Chlamydomonas reinhardtii am Beispiel des Cre/lox-Systems. PhD, Universität Regensburg

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Abstract (German)

Die Grünalge Chlamydomonas reinhardtii hat sich in den vergangenen Jahrzehnten als Modellorganismus für diverse zellbiologische Problemstellungen etabliert. Es wurde eine Vielzahl von Methoden entwickelt, welche die Transformation des Algengenoms erlauben. Wie auch in höheren Pflanzen findet die Integration rekombinanter DNA nichthomolog, d.h. ungerichtet im Genom statt. Dies bedeutet, dass ...

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Translation of the abstract (English)

The green alga Chlamydomonas reinhardtii has been established as a model organism for fundamental biological research during the last decades. Several reliable transformation methods have been developed to manipulate the nuclear genome, but similar to higher plants the integration of DNA occurs predominantly via nonhomologous recombination at apparently random loci. As a consequence the ...

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Item Type:Thesis of the University of Regensburg (PhD)
Date:19 October 2008
Referee:Peter (Prof. Dr.) Hegemann
Date of exam:20 July 2007
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie
Keywords:Rekombination , Homologe Rekombination , Heterologe Genexpression , Transformation <Genetik> , Integration <Genetik> , Chlamydomonas reinhardii , ortsgerichtete Rekombination , Cre/lox System , Selektionsmarker , site-specific recombination , Cre/lox system , transformation , heterologous gene expression
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Deposited On:27 Oct 2009 16:17
Last Modified:13 Mar 2014 11:51
Item ID:10687
Owner Only: item control page

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