Stage-specific antigens reacting with monoclonal antibodies against contact site A, a cell-surface glycoprotein of Dictyostelium discoideum

Abstract

Monoclonal antibodies against a glycoprotein presumably involved in adhesion of aggregating Dictyostelium discoideum cells have been used for labeling of the antigen at the cell surface. The antigen is distributed over the whole surface of the cells, apparently in form of small clusters. The antigen appears concomitantly with the acquisition of EDTA-stable adhesiveness typical of aggregation competent cells. In contrast, discoidin I, a lectin whose accumulation during development parallels EDTA-stable adhesiveness in another strain (NC-4), is present in nearly the same amounts of growth phase and aggregating cells of AX2-214, the strain used by use. Thus, no correlation exists in this strain between the expression of discoidin I and the development of cell adhesiveness. The 80 kilodalton glycoprotein typical of aggregation competent cells has been purified by affinity chromatography on a monoclonal antibody column. The purified antigen absorbs adhesion-blocking Fab from rabbits. Another antigen strongly reacting with the same monoclonal antibodies has an apparent molecular weight of 106 000 and is not detectable before slugs are formed.