Sander, Chris and Vriend, Gerrit and Bazan, Fernando and Horovitz, Amnon and Nakamura, Haruki and Ribas, Luis and Finkelstein, Alexei V. and Lockhart, Andrew and Merkl, Rainer and Perry, L. Jeanne and Emery, Stephen C. and Gaboriaud, Christine and Marks, Cara and Moult, John and Verlinde, Christophe and Eberhard, Marc and Elofsson, Arne and Hubbard, Tim J. P. and Regan, Lynne and Banks, Jay and Jappelli, Roberto and Lesk, Arthur M. and Tramontano, Anna (1992) Protein design on computers. Five new proteins: Shpilka, Grendel, Fingerclasp, Leather, and Aida. Proteins 12 (2), pp. 105-110.
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What is the current state of the art in protein design? This question was approached in a recent two-week protein design workshop sponsored by EMBO and held at the EMBL in Heidelberg. The goals were to test available design tools and to explore new design strategies. Five novel proteins were designed: Shpilka, a sandwich of two four-stranded beta-sheets, a scaffold on which to explore variations in loop topology; Grendel, a four-helical membrane anchor, ready for fusion to water-soluble functional domains; Finger-clasp, a dimer of interdigitating beta-beta-alpha units, the simplest variant of the "handshake" structural class; Aida, an antibody binding surface intended to be specific for flavodoxin; Leather--a minimal NAD binding domain, extracted from a larger protein. Each design is available as a set of three-dimensional coordinates, the corresponding amino acid sequence and a set of analytical results. The designs are placed in the public domain for scrutiny, improvement, and possible experimental verification.
|Institutions:||Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner > Arbeitsgruppe PD Dr. Rainer Merkl|
|Keywords:||Algorithms Amino Acid Sequence Carrier Proteins/*chemistry/metabolism Computers Flavodoxin/metabolism Molecular Sequence Data NAD/metabolism Protein Conformation *Protein Engineering Proteins/*chemistry/metabolism Support, Non-U.S. Gov't|
|Subjects:||500 Science > 570 Life sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Deposited On:||16 Nov 2009 10:59|
|Last Modified:||21 Dec 2009 08:46|
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