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A robust protein host for anchoring chelating ligands and organocatalysts.

Reetz, Manfred T. and Rentzsch, Martin and Pletsch, Andreas and Taglieber, Andreas and Hollmann, Frank and Mondière, Régis J. G. and Dickmann, Norbert and Höcker, Birte and Cerrone, Simona and Haeger, Michaela C. and Sterner, Reinhard (2008) A robust protein host for anchoring chelating ligands and organocatalysts. Chembiochem : a European journal of chemical biology 9 (4), pp. 552-64.

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Abstract

In order to put the previously proposed concept of directed evolution of hybrid catalysts (proteins that harbor synthetic transition-metal catalysts or organocatalysts) into practice, several prerequisites must be met. The availability of a robust host protein that can be expressed in sufficiently large amounts, and that can be purified in a simple manner is crucial. The thermostable enzyme tHisF ...

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Item Type:Article
Date:2008
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner
Identification Number:
ValueType
18273849PubMed ID
10.1002/cbic.200700413DOI
Classification:
NotationType
CatalysisMESH
Chelating Agents/chemistryMESH
Gene ExpressionMESH
LigandsMESH
Maleimides/chemistryMESH
Models, MolecularMESH
Molecular StructureMESH
Mutation/geneticsMESH
Protein BindingMESH
Proteins/metabolismMESH
Spectrometry, Mass, Electrospray IonizationMESH
Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:22 Mar 2010 07:46
Last Modified:22 Mar 2010 09:46
Item ID:13670
Owner Only: item control page
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