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A mammalian expression vector for the expression of GAL4 fusion proteins with an epitope tag and histidine tail

Witzgall, Ralph and O'Leary, Eileen and Bonventre, Joseph V. (1994) A mammalian expression vector for the expression of GAL4 fusion proteins with an epitope tag and histidine tail. Analytical Biochemistry 223 (2), pp. 291-298.

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Abstract

Expression of newly cloned cDNAs in mammalian cell lines is an essential tool for the functional analysis of the proteins encoded by these cDNAs. In many instances, however, evaluation of the protein is difficult because of the difficulty in purification of the expressed protein and/or the lack of specific antibodies which react with the proteins on Western blots or for immunocytochemistry or ...

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Item Type:Article
Date:1994
Institutions:Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und Zelluläre Anatomie > Prof. Dr. Ralph Witzgall
Research groups and research centres:Not selected
Identification Number:
ValueType
10.1006/abio.1994.1587 DOI
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:No
Owner: Universitätsbibliothek Regensburg
Deposited On:06 Dec 2010 09:14
Last Modified:13 Mar 2014 14:14
Item ID:15900
Owner Only: item control page

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