Muckenschnabel, Ingo and Bernhardt, Günther and Spruß, Thilo and Dietl, Barbara and Buschauer, Armin (1998) Quantitation of hyaluronidases by the Morgan-Elson reaction: comparison of the enzyme activities in the plasma of tumor patients and healthy volunteers. Cancer Letters 131, pp. 13-20.
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Other URL: doi:10.1016/S0304-3835%2898%2900196-7
The Morgan-Elson reaction, a method for the detn. of hyaluronidase activity, was optimized for the quantitation of the enzyme in biol. material. Based on HPLC and spectrometric (UV-Vis, LC-MS) studies, the structure of the red-colored product (mesomeric forms of N3-protonated 3-acetylimino-2-(4-dimethylaminophenyl)methylidene-5-(1,2-dihydroxyethyl)furane) formed by condensation of chromogen III with p-dimethylaminobenzaldehyde is proposed. Activities corresponding to ³0.1 IU of endogenous and therapeutically administered hyaluronidase can be detected in 50 ml samples. Application of the method for the detn. of the enzyme in plasma of tumor patients revealed no difference in activity levels, interindividual variability and pH profile compared to healthy volunteers.
|Institutions:||Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)|
|Subjects:||500 Science > 570 Life sciences|
600 Technology > 610 Medical sciences Medicine
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Owner:||Prof. Armin Buschauer|
|Deposited On:||22 Feb 2008 13:18|
|Last Modified:||05 Aug 2009 13:42|