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Genetische Transformation von Volvox carteri mit einem dominant-selektiven Resistenzmarker

Jakobiak, Thomas (2004) Genetische Transformation von Volvox carteri mit einem dominant-selektiven Resistenzmarker. PhD, Universität Regensburg.

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Date of publication of this fulltext: 08 Jan 2004 15:32

Abstract (German)

Das Aminoglykosid-Antibiotikum Paromomycin, welches hoch toxisch für die Grünalge Volvox carteri ist, wird durch die Aminoglykosid-3'-Phosphotransferase (APHVIII) aus Streptomyces rimosus effektiv inaktiviert. In dieser Arbeit wurde das bakterielle aphH Gen, das dieses Enzym kodiert genetisch für die Expression in Volvox bearbeitet, um dieser Alge eine Paromomycin Resistenz zu vermitteln. Die ...


Translation of the abstract (English)

The amino-glycoside antibiotic paromomycin that is highly toxic to the green alga Volvox carteri is efficiently inactivated by aminoglycoside-3'-phosphotransferase from Streptomyces rimosus. In an attempt to develop a new dominant selectable marker � paromomycin resistance (PmR) � for use in Volvox nuclear transformation we made different constructs in which the bacterial aphH gene encoding this ...


Export bibliographical data

Item type:Thesis of the University of Regensburg (PhD)
Date:7 January 2004
Referee:Rüdiger (Prof. Dr.) Schmitt
Date of exam:22 December 2003
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie
Keywords:Transformation <Genetik> , Volvox , Grünalgen , Heterologe Genexpression , Dominanter Resistenz-Marker , transformation , genetics , Volvox , green algae , heterologous marker , resistance
Dewey Decimal Classification:500 Science > 570 Life sciences
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:10155
Owner only: item control page


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