Quantitative Analysis of Cell Motility and Chemotaxis in Dictyostelium discoideum by Using an Image Processing System and a Novel Chemotaxis Chamber Providing Stationary Chemical Gradients

URN to cite this document:

urn:nbn:de:bvb:355-epub-109084 Copy

DOI to cite this document:

10.5283/epub.10908 Copy

Fisher, P. R. ; Merkl, Rainer ; Gerisch, G.

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Date of publication of this fulltext: 13 Nov 2009 13:34

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Details

Item type:	Article
Journal or Publication Title:	The Journal of Cell Biology (JCB)
Publisher:	Rockefeller University Press
Volume:	108
Number of Issue or Book Chapter:	3
Page Range:	pp. 973-984
Date:	1989
Institutions:	Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl
Identification Number:	Value Type 2537839 PubMed ID
Classification:	Notation Type Cell Movement MESH Chemotaxis* MESH Cyclic AMP/pharmacology MESH Dictyostelium/growth & development MESH Dictyostelium/physiology* MESH Image Processing, Computer-Assisted MESH Microscopy, Phase-Contrast MESH
Keywords:	Cell Movement *Chemotaxis Cyclic AMP/pharmacology Dictyostelium/growth & development/*physiology Image Processing, Computer-Assisted Microscopy, Phase-Contrast Support, Non-U.S. Gov't
Dewey Decimal Classification:	500 Science > 570 Life sciences
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	No
Item ID:	10908

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Abstract

An image processing system was programmed to automatically track and digitize the movement of amebae under phase-contrast microscopy. The amebae moved in a novel chemotaxis chamber designed to provide stable linear attractant gradients in a thin agarose gel. The gradients were established by pumping attractant and buffer solutions through semipermeable hollow fibers embedded in the agarose gel. ...

Abstract

An image processing system was programmed to automatically track and digitize the movement of amebae under phase-contrast microscopy. The amebae moved in a novel chemotaxis chamber designed to provide stable linear attractant gradients in a thin agarose gel. The gradients were established by pumping attractant and buffer solutions through semipermeable hollow fibers embedded in the agarose gel. Gradients were established within 30 min and shown to be stable for at least a further 90 min. By using this system it is possible to collect detailed data on the movement of large numbers of individual amebae in defined attractant gradients. We used the system to study motility and chemotaxis by a score of Dictyostelium discoideum wild-type and mutant strains, including "streamer" mutants which are generally regarded as being altered in chemotaxis. None of the mutants were altered in chemotaxis in the optimal cAMP gradient of 25 nM/mm, with a midpoint of 25 nM. The dependence of chemotaxis on cAMP concentration, gradient steepness, and temporal changes in the gradient were investigated. We also analyzed the relationship between turning behavior and the direction of travel during chemotaxis in stable gradients. The results suggest that during chemotaxis D. discoideum amebae spatially integrate information about local increases in cAMP concentration at various points on the cell surface.

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Metadata last modified: 26 Nov 2020 12:14

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