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Quantitative Analysis of Cell Motility and Chemotaxis in Dictyostelium discoideum by Using an Image Processing System and a Novel Chemotaxis Chamber Providing Stationary Chemical Gradients

Fisher, P. R., Merkl, Rainer and Gerisch, G. (1989) Quantitative Analysis of Cell Motility and Chemotaxis in Dictyostelium discoideum by Using an Image Processing System and a Novel Chemotaxis Chamber Providing Stationary Chemical Gradients. The Journal of Cell Biology (JCB) 108 (3), pp. 973-984.

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Date of publication of this fulltext: 13 Nov 2009 13:34

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Other URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115405/?tool=pubmed


Abstract

An image processing system was programmed to automatically track and digitize the movement of amebae under phase-contrast microscopy. The amebae moved in a novel chemotaxis chamber designed to provide stable linear attractant gradients in a thin agarose gel. The gradients were established by pumping attractant and buffer solutions through semipermeable hollow fibers embedded in the agarose gel. ...

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Item type:Article
Date:1989
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl
Identification Number:
ValueType
2537839PubMed ID
Classification:
NotationType
Cell MovementMESH
Chemotaxis*MESH
Cyclic AMP/pharmacologyMESH
Dictyostelium/growth & developmentMESH
Dictyostelium/physiology*MESH
Image Processing, Computer-AssistedMESH
Microscopy, Phase-ContrastMESH
Keywords:Cell Movement *Chemotaxis Cyclic AMP/pharmacology Dictyostelium/growth & development/*physiology Image Processing, Computer-Assisted Microscopy, Phase-Contrast Support, Non-U.S. Gov't
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:No
Item ID:10908
Owner only: item control page

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