Gläsner, W. ; Merkl, Rainer ; Schellenberger, V. ; Fritz, Hans Joachim
Alternative Links zum Volltext:PubmedVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Journal of Molecular Biology |
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| Verlag: | Elsevier |
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| Band: | 245 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 1 |
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| Seitenbereich: | S. 1-7 |
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| Datum: | 1995 |
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| Institutionen: | Biologie und Vorklinische Medizin > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl |
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| Identifikationsnummer: | |
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| Klassifikation: | | Notation | Art |
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| Base Sequence | MESH | | DNA Repair/genetics | MESH | | Deoxyribonuclease I/genetics* | MESH | | Deoxyribonuclease I/metabolism | MESH | | Escherichia coli/enzymology | MESH | | Escherichia coli/genetics* | MESH | | Evolution | MESH | | Fluorescent Dyes | MESH | | Genome, Bacterial* | MESH | | Molecular Sequence Data | MESH | | Mutagenesis | MESH | | Oligonucleotides/analysis | MESH | | Substrate Specificity/genetics | MESH |
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| Stichwörter / Keywords: | Base Sequence DNA Repair/genetics Deoxyribonuclease I/*genetics/metabolism Escherichia coli/enzymology/*genetics Evolution Fluorescent Dyes *Genome, Bacterial Molecular Sequence Data Mutagenesis Oligonucleotides/analysis Substrate Specificity/genetics Support, Non-U.S. Gov't |
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| Dewey-Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Nein |
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| Dokumenten-ID: | 10910 |
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Zusammenfassung
The substrate spectrum of Vsr DNA mismatch endonuclease of Escherichia coli K-12 was investigated using fluorescence-labelled oligonucleotide substrates and a DNA sequencer for detection and quantification of substrates and reaction products. Fourteen substrates were found to be processed by the enzyme, which differ in one or two positions from the canonical pentanucleotide sequence CTA/TGG (T ...
Zusammenfassung
The substrate spectrum of Vsr DNA mismatch endonuclease of Escherichia coli K-12 was investigated using fluorescence-labelled oligonucleotide substrates and a DNA sequencer for detection and quantification of substrates and reaction products. Fourteen substrates were found to be processed by the enzyme, which differ in one or two positions from the canonical pentanucleotide sequence CTA/TGG (T mismatched to G). Relative second-order rate constants of these substrates were determined in groups of four by multiple substrate kinetics and compared to the underresentation of the corresponding pentanucleotides in the E. coli K-12 genome. The high quality of correlation further establishes active mutagenesis by VSP repair as a significant driving force of the evolution of the E. coli K-12 genome and provides clues to its possible selective value.
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