| Item type: | Article | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Journal or Publication Title: | The Journal of Biological Chemistry | ||||||||||||||||||||||||
| Publisher: | American Society for Biochemistry and Molecular Biology | ||||||||||||||||||||||||
| Volume: | 262 | ||||||||||||||||||||||||
| Number of Issue or Book Chapter: | 34 | ||||||||||||||||||||||||
| Page Range: | pp. 16618-16624 | ||||||||||||||||||||||||
| Date: | 1987 | ||||||||||||||||||||||||
| Institutions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl | ||||||||||||||||||||||||
| Identification Number: |
| ||||||||||||||||||||||||
| Classification: |
| ||||||||||||||||||||||||
| Keywords: | Antibodies, Monoclonal Biological Transport, Active Carbohydrates/analysis Concanavalin A/metabolism Dictyostelium/*analysis Fluorescent Antibody Technique Glycosylation Molecular Weight Support, Non-U.S. Gov't Surface Properties Viral Proteins/*metabolism Wheat Germ Agglutinins/metabolism | ||||||||||||||||||||||||
| Dewey Decimal Classification: | 500 Science > 570 Life sciences | ||||||||||||||||||||||||
| Status: | Published | ||||||||||||||||||||||||
| Refereed: | Yes, this version has been refereed | ||||||||||||||||||||||||
| Created at the University of Regensburg: | No | ||||||||||||||||||||||||
| Item ID: | 10916 |
Abstract
Two different types of oligosaccharides, designated type 1 and 2 carbohydrate residues, are present on the contact site A molecule, an 80-kDa glycoprotein involved in the formation of EDTA-stable cell adhesion during cell aggregation in Dictyostelium discoideum. The first precursor detected by pulse-chase labeling with [35S]methionine was a 68-kDa glycoprotein carrying type 1 carbohydrate. ...
Abstract
Two different types of oligosaccharides, designated type 1 and 2 carbohydrate residues, are present on the contact site A molecule, an 80-kDa glycoprotein involved in the formation of EDTA-stable cell adhesion during cell aggregation in Dictyostelium discoideum. The first precursor detected by pulse-chase labeling with [35S]methionine was a 68-kDa glycoprotein carrying type 1 carbohydrate. Conversion of the precursor into the 80-kDa form occurred simultaneously with the addition of type 2 carbohydrate. Tunicamycin inhibited type 1 glycosylation more efficiently than type 2 glycosylation. The first precursor detected in tunicamycin-treated cells by pulse-chase labeling was a 53-kDa protein lacking both carbohydrates, which was converted through addition of type 2 carbohydrate into a 66-kDa final product. Labeling of intact cells indicated that this 66-kDa glycoprotein is transported to the cell surface. Prolonged treatment with tunicamycin resulted in the accumulation within the cells of the 53-kDa precursor with no detectable exposure of this protein on the cell surface. It is concluded that type 1 carbohydrate, which is cotranslationally added in N-glycosidic linkages, is neither required for transport of the protein to the Golgi apparatus nor for type 2 glycosylation or protection of the protein against proteolytic degradation. Incapability of tunicamycin-treated cells of forming EDTA-stable cell contacts suggests a role for type 1 carbohydrate in cell adhesion. Type 2 carbohydrate is added posttranslationally. It is required in the absence of type 1 glycosylation for transport of the protein to the cell surface.
Metadata last modified: 24 May 2018 11:42
Altmetric