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Two-step Glycosylation of the Contact Site A Protein of Dictyostelium discoideum and Transport of an Incompletely Glycosylated Form to the Cell Surface

Hohmann, H. P. ; Bozzaro, S. ; Yoshida, M. ; Merkl, Rainer ; Gerisch, G.



Abstract

Two different types of oligosaccharides, designated type 1 and 2 carbohydrate residues, are present on the contact site A molecule, an 80-kDa glycoprotein involved in the formation of EDTA-stable cell adhesion during cell aggregation in Dictyostelium discoideum. The first precursor detected by pulse-chase labeling with [35S]methionine was a 68-kDa glycoprotein carrying type 1 carbohydrate. ...

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