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Monoclonal anti-glycoprotein antibody that blocks cell adhesion in Polysphondylium pallidum

Toda, Katsumi ; Bozzaro, Salvatore ; Lottspeich, Friedrich ; Merkl, Rainer ; Gerisch, Günther



Abstract

Polyclonal and monoclonal antibodies were prepared against a glycoprotein (gp 64) of Polysphondylium pallidum previously shown to act as a target site of adhesion-blocking Fab prepared from antisera against whole membranes of aggregation-competent cells. The purified glycoprotein, with a nominal Mr of 64000, could be fractionated into two subspecies, gp 64I and gp 64II, with apparent Mr of 66000 ...

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