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Functional annotation and three-dimensional structure of Dr0930 from Deinococcus radiodurans, a close relative of phosphotriesterase in the amidohydrolase superfamily.

Xiang, Dao Feng ; Kolb, Peter ; Fedorov, Alexander A. ; Meier, Monika M. ; Fedorov, Lena V. ; Nguyen, T. Tinh ; Sterner, Reinhard ; Almo, Steven C. ; Shoichet, Brian K. ; Raushel, Frank M.



Abstract

Dr0930, a member of the amidohydrolase superfamily in Deinococcus radiodurans, was cloned, expressed, and purified to homogeneity. The enzyme crystallized in the space group P3121, and the structure was determined to a resolution of 2.1 A. The protein folds as a (beta/alpha)7beta-barrel, and a binuclear metal center is found at the C-terminal end of the beta-barrel. The purified protein contains ...

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