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Schwab, Thomas ; Skegro, Darko ; Mayans, Olga ; Sterner, Reinhard

A rationally designed monomeric variant of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus is as active as the dimeric wild-type enzyme but less thermostable.

Schwab, Thomas, Skegro, Darko, Mayans, Olga and Sterner, Reinhard (2008) A rationally designed monomeric variant of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus is as active as the dimeric wild-type enzyme but less thermostable. Journal of molecular biology 376 (2), pp. 506-16.

Date of publication of this fulltext: 22 Mar 2010 07:49
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Item typeArticle
Journal or Publication TitleJournal of molecular biology
Publisher:ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Place of Publication:LONDON
Volume:376
Number of Issue or Book Chapter:2
Page Range:pp. 506-16
Date2008
InstitutionsBiology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner
Identification Number
ValueType
18164726PubMed ID
10.1016/j.jmb.2007.11.078DOI
Classification
NotationType
Anthranilate Phosphoribosyltransferase/metabolismMESH
Calorimetry, Differential ScanningMESH
CatalysisMESH
Crystallography, X-RayMESH
DimerizationMESH
Enzyme StabilityMESH
Escherichia coli/geneticsMESH
Genetic VariationMESH
Hot TemperatureMESH
Hydrogen BondingMESH
HydrophobicityMESH
KineticsMESH
Models, MolecularMESH
MutationMESH
PlasmidsMESH
Protein Structure, SecondaryMESH
Substrate SpecificityMESH
Sulfolobus solfataricus/enzymologyMESH
KeywordsQUINOLINIC ACID PHOSPHORIBOSYLTRANSFERASE; CRYSTAL-STRUCTURE; MYCOBACTERIUM-TUBERCULOSIS; TRIOSEPHOSPHATE ISOMERASE; OVERLAP EXTENSION; DOUBLE MUTATION; PROTEIN; STABILITY; OLIGOMERIZATION; PHOSPHORYLASE; anthranilate phosphoribosyltransferase; hyperthermophile; rational protein design; sulfolobus solfataricus; thermal stability
Dewey Decimal Classification500 Science > 570 Life sciences
StatusPublished
RefereedYes, this version has been refereed
Created at the University of RegensburgYes
Item ID13671

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