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Evolution of multi-enzyme complexes: the case of tryptophan synthase.

Leopoldseder, Sonja ; Hettwer, Stefan ; Sterner, Reinhard


The prototypical tryptophan synthase is a stable heterotetrameric alpha-betabeta-alpha complex. The constituting TrpA and TrpB1 subunits, which are encoded by neighboring genes in the trp operon, activate each other in a bi-directional manner. Recently, a novel class of TrpB2 proteins has been identified, whose members contain additional amino acids that might sterically prevent complex formation ...


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