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Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis.

Thoma, Ralf ; Obmolova, G. ; Lang, D. A. ; Schwander, M. ; Jenö, P. ; Sterner, Reinhard ; Wilmanns, Matthias



Abstract

Enzymes from hyperthermophiles can be efficiently purified after expression in mesophilic hosts and are well-suited for crystallisation attempts. Two enzymes of histidine biosynthesis from Thermotoga maritima, N'-((5'-phosphoribosyl)-formimino)-5-aminoimidazol-4-carb oxamid ribonucleotide isomerase and the cyclase moiety of imidazoleglycerol phosphate synthase, were overexpressed in Escherichia ...

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