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Phosphoribosyl anthranilate isomerase from Thermotoga maritima is an extremely stable and active homodimer.

Sterner, Reinhard ; Kleemann, G. R. ; Szadkowski, H. ; Lustig, A. ; Hennig, Michael ; Kirschner, Kasper


The metabolism of hyperthermophilic microorganisms can function properly at temperatures close to 100 degrees C. It follows that they are equipped with both thermostable enzymes and mechanisms that handle labile metabolites. We wanted to understand how stable and active phosphoribosyl anthranilate isomerase (tPRAI) from the hyperthermophile Thermotoga maritima is at its optimum growth temperature ...


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