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DNA sequence encoded repression of rRNA gene transcription in chromatin

Felle, Max ; Exler, Josef H. ; Merkl, Rainer ; Dachauer, Karoline ; Brehm, Alexander ; Grummt, Ingrid ; Längst, Gernot



Abstract

Eukaryotic genomes are packaged into nucleosomes that occlude DNA from interacting with most DNA-binding proteins. Nucleosome positioning and chromatin organization is critical for gene regulation. We have investigated the mechanism by which nucleosomes are positioned at the promoters of active and silent rRNA genes (rDNA). The reconstitution of nucleosomes on rDNA results in sequence-dependent ...

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