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Cu2+-cyclen as Probe to Identify Conformational States in Guanine Nucleotide Binding Proteins

Meierhofer, T. ; Rosnizeck, I. C. ; Graf, Thorsten ; Reiss, K. ; Kalbitzer, Hans Robert ; Spörner, Michael ; König, Burkhard


(31)P NMR spectroscopy is a suitable method for identifying conformational states in the active site of guanine nucleotide binding proteins detecting the nucleotide placed there. Because there is no labeling necessary, this method is gaining increasing interest. By (31)P NMR spectroscopy two major conformational states, namely state 1(T) and state 2(T), can be detected in active Ras protein ...


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