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A Nanogel for Ratiometric Fluorescent Sensing of Intracellular pH Values

DOI to cite this document:
Peng, Hong-shang ; Stolwijk, Judith A. ; Sun, Li-Ning ; Wegener, Joachim ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 18 Mar 2011 06:53


A ratiometric fluorescent nanogel can sense pH over a range that is applicable to physiological studies. It can be easily prepared and made pH-responsive by addition of a pH probe and a FRET system that utilizes the gel to hold dyes in close proximity (see picture; overlay of coumarin dye and Nile Red fluorescence in kidney cells).

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