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Ligand-induced formation of a transient tryptophan synthase complex with aßß subunit stoichiometry

Ehrmann, Alexander ; Richter, Klaus ; Busch, Florian ; Reimann, Julia ; Albers, Sonja-Verena ; Sterner, Reinhard


The prototypical tryptophan synthases form a stable heterotetrameric RββR complex in which the constituting TrpA and TrpB1 subunits activate each other in a bidirectional manner. The hyperthermophilic archaeon Sulfolobus solfataricus does not contain a TrpB1 protein but instead two members of the phylogenetically distinct family of TrpB2 proteins, which are encoded within (sTrpB2i) and ...


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