Enhancing the stability and solubility of the glucocorticoid receptor ligand-binding domain by high-throughput library screening

Seitz, Tobias, Thoma, Ralf, Schoch, Guillaume A., Stihle, Martine, Benz, Jörg, D'Arcy, Brigitte, Wiget, Andrea, Ruf, Armin, Hennig, Michael and Sterner, Reinhard (2010) Enhancing the stability and solubility of the glucocorticoid receptor ligand-binding domain by high-throughput library screening. Journal of molecular biology 403 (4), pp. 562-577.

Date of publication of this fulltext: 03 May 2011 06:01
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• Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner

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Details

Item type	Article
Journal or Publication Title	Journal of molecular biology
Publisher:	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Place of Publication:	LONDON
Volume:	403
Number of Issue or Book Chapter:	4
Page Range:	pp. 562-577
Date	2010
Institutions	Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner
Identification Number	Value Type 20850457 PubMed ID 10.1016/j.jmb.2010.08.048 DOI
Classification	Notation Type Amino Acid Sequence MESH Amino Acid Substitution MESH Animals MESH Base Sequence MESH Binding Sites/genetics MESH Crystallography, X-Ray MESH Escherichia coli/genetics MESH Flow Cytometry MESH Gene Library MESH Green Fluorescent Proteins/genetics MESH Humans MESH Ligands MESH Mice MESH Models, Molecular MESH Molecular Sequence Data MESH Mutagenesis MESH Protein Conformation MESH Protein Engineering MESH Protein Stability MESH Protein Structure, Tertiary MESH Receptors, Glucocorticoid/genetics MESH Recombinant Fusion Proteins/genetics MESH Sequence Homology, Amino Acid MESH Solubility MESH Static Electricity MESH Tumor Necrosis Factor Ligand Superfamily Member 13/genetics MESH
Keywords	GREEN FLUORESCENT PROTEIN; DIRECTED ENZYME EVOLUTION; RAY CRYSTAL-STRUCTURE; HETEROLOGOUS EXPRESSION; MUTAGENESIS; THERMOSTABILITY; MECHANISMS; ANTAGONISM; SUFFICIENT; SOFTWARE; FACS; GFP; folding reporter; library screening; glucocorticoid receptor
Dewey Decimal Classification	500 Science > 570 Life sciences
Status	Published
Refereed	Yes, this version has been refereed
Created at the University of Regensburg	Yes
Item ID	20721

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