Nucleosomes protect DNA from DNA methylation in vivo and in vitro

URN to cite this document:: urn:nbn:de:bvb:355-epub-216230
DOI to cite this document:: 10.5283/epub.21623

Felle, Max ; Hoffmeister, Helen ; Rothammer, Julia ; Fuchs, Andreas ; Exler, Josef H. ; Längst, Gernot

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Date of publication of this fulltext: 26 Jul 2011 08:03

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Abstract

Positioned nucleosomes limit the access of proteins to DNA. However, the impact of nucleosomes on DNA methylation in vitro and in vivo is poorly understood. Here, we performed a detailed analysis of nucleosome binding and nucleosomal DNA methylation by the de novo methyltransferases. We show that compared to linker DNA, nucleosomal DNA is largely devoid of CpG methylation. ATP-dependent chromatin ...