Generation of specific antibodies against the rap1A, rap1B and rap2 small GTP-binding proteins. Analysis of rap and ras proteins in membranes from mammalian cells

URN to cite this document:: urn:nbn:de:bvb:355-epub-232202
DOI to cite this document:: 10.5283/epub.23220

Klinz, F. J. ; Seifert, Roland ; Schwaner, I. ; Gausepohl, H. ; Frank, R. ; Schultz, Günter

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Date of publication of this fulltext: 24 Jan 2012 07:57

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Abstract

Specific antibodies against rap1A and rap1B small GTP-binding proteins were generated by immunization of rabbits with peptides derived from the C-terminus of the processed proteins. Immunoblot analysis of membranes from several mammalian cell lines and human thrombocytes with affinity-purified antibodies against rap1A or rap1B demonstrated the presence of multiple immunoreactive proteins in the ...