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Generation of specific antibodies against the rap1A, rap1B and rap2 small GTP-binding proteins. Analysis of rap and ras proteins in membranes from mammalian cells

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Klinz, F. J. ; Seifert, Roland ; Schwaner, I. ; Gausepohl, H. ; Frank, R. ; Schultz, Günter
Date of publication of this fulltext: 24 Jan 2012 07:57


Specific antibodies against rap1A and rap1B small GTP-binding proteins were generated by immunization of rabbits with peptides derived from the C-terminus of the processed proteins. Immunoblot analysis of membranes from several mammalian cell lines and human thrombocytes with affinity-purified antibodies against rap1A or rap1B demonstrated the presence of multiple immunoreactive proteins in the ...


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