Offermanns, S., Seifert, Roland, Metzger, J. W., Jung, G., Lieberknecht, A., Schmidt, U. and Schultz, Günter
(1992)
Lipopeptides are effective stimulators of tyrosine phosphorylation in human myeloid cells.
Biochemical Journal 282 ( Pt 2, pp. 551-7.
Date of publication of this fulltext: 24 Jan 2012 08:04at PubMed
Abstract
Synthetic lipopeptide analogues of the N-terminus of bacterial lipoprotein are effective activators of macrophages, neutrophils and lymphocytes. We studied the effect of the lipopeptide N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]- (R)-cysteinyl-(S)-seryl-(S)-lysyl-(S)-lysyl-(S)-lysyl-(S)-lysine [Pam3Cys-Ser-(Lys)4] on tyrosine phosphorylation in dibutyryl-cyclic-AMP-differentiated HL-60 ...
Abstract
Synthetic lipopeptide analogues of the N-terminus of bacterial lipoprotein are effective activators of macrophages, neutrophils and lymphocytes. We studied the effect of the lipopeptide N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]- (R)-cysteinyl-(S)-seryl-(S)-lysyl-(S)-lysyl-(S)-lysyl-(S)-lysine [Pam3Cys-Ser-(Lys)4] on tyrosine phosphorylation in dibutyryl-cyclic-AMP-differentiated HL-60 cells, using anti-phosphotyrosine antibodies. Pam3Cys-Ser-(Lys)4 concentration-dependently stimulated tyrosine phosphorylation of 100/110 kDa and 60 kDa proteins and, to a lesser extent, of 55 kDa and 70/75 kDa proteins. Half-maximal and maximal effects were observed at concentrations of 1-6 and 5-50 micrograms/ml respectively. The lipopeptide-induced increase in phosphorylation was rapid and transient, with a peak response after 30-60 s. The lipopeptide (2S)-2-palmitoylamino-6-palmitoyloxymethyl-7-palmitoyloxy heptanoyl-Ser-(Lys)4 [Pam3Ahh-Ser-(Lys)4] was as potent as Pam3Cys-Ser(Lys)4, whereas (2S,6S)-2-palmitoylamino-6,7-bis(palmitoyloxy)heptanoyl++ +-Ser-(Lys)4 [Pam3Adh-Ser-(Lys)4] and Pam3Cys-Ser-Gly did not induce tyrosine phosphorylation. Lipopeptide-induced tyrosine phosphorylation was not affected by treatment of cells with pertussis toxin. Neither phorbol 12-myristate 13-acetate nor A23187 induced tyrosine phosphorylation in dibutyryl-cyclic-AMP-differentiated HL-60 cells. In HL-60 promyelocytes, Pam3Cys-Ser-(Lys)4 had no effect on tyrosine phosphorylation, whereas the lipopeptide also induced tyrosine phosphorylation in 1,25-dihydroxyvitamin-D3-differentiated HL-60 cells and in human neutrophils. These results show that lipopeptides are effective stimulators of tyrosine phosphorylation in mature human myeloid cells.
Export bibliographical data
| Item type: | Article |
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| Date: | 1992 |
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| Institutions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmacology and Toxicology (Prof. Schlossmann, formerly Prof. Seifert) |
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| Identification Number: | | Value | Type |
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| 2625355 | PubMed ID |
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| Classification: | | Notation | Type |
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| Blotting, Western | MESH | | Bucladesine/pharmacology | MESH | | Cell Differentiation/drug effects | MESH | | Cells, Cultured | MESH | | Electrophoresis, Polyacrylamide Gel | MESH | | Genistein | MESH | | Humans | MESH | | Isoflavones/pharmacology | MESH | | Lipoproteins/pharmacology | MESH | | Neutrophils/metabolism | MESH | | Pertussis Toxin | MESH | | Phosphorylation/drug effects | MESH | | Tetradecanoylphorbol Acetate/pharmacology | MESH | | Tyrosine/metabolism | MESH | | Virulence Factors, Bordetella/pharmacology | MESH |
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| Dewey Decimal Classification: | 600 Technology > 615 Pharmacy |
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| Status: | Published |
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| Refereed: | Yes, this version has been refereed |
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| Created at the University of Regensburg: | Unknown |
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| Item ID: | 23221 |
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