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Comparative determination of cefotaxime and desacetyl cefotaxime in serum and bile by bioassay and high-performance liquid chromatography

URN to cite this document:
urn:nbn:de:bvb:355-epub-268607
Kees, Frieder K. ; Strehl, E. ; Seeger, K. ; Seidel, G. ; Dominiak, P. ; Grobecker, H.
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Date of publication of this fulltext: 29 Nov 2012 12:33


Abstract

In rat serum as well as in human serum and bile after injection of cefotaxime (CTX), the parent compound and the active metabolite desacetyl cefotaxime (dCTX) have been demonstrated by quantitative analysis with high-performance liquid chromatography (HPLC). Simultaneous determination of CTX by bioassay using test organisms sensitive to both CTX and dCTX resulted in spuriously high concentration ...

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