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RNA “Traffic Lights”: An Analytical Tool to Monitor siRNA Integrity

Holzhauser, C., Liebl, Renate, Goepferich, Achim, Wagenknecht, Hans-Achim and Breunig, Miriam (2013) RNA “Traffic Lights”: An Analytical Tool to Monitor siRNA Integrity. ACS chemical biology 8, pp. 890-894.

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The combination of thiazole orange and thiazole red as an internal energy transfer-based fluorophore pair in oligonucleotides provides an outstanding analytical tool to follow DNA/RNA hybridization through a distinct fluorescence color change from red to green. Herein, we demonstrate that this concept can be applied to small interfering RNA (siRNA) to monitor RNA integrity in living cells in real ...


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Item type:Article
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich)
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Dewey Decimal Classification:600 Technology > 615 Pharmacy
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:29275
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