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RNA “Traffic Lights”: An Analytical Tool to Monitor siRNA Integrity

Holzhauser, C., Liebl, Renate, Goepferich, Achim, Wagenknecht, Hans-Achim and Breunig, Miriam (2013) RNA “Traffic Lights”: An Analytical Tool to Monitor siRNA Integrity. ACS chemical biology 8, pp. 890-894.

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Abstract

The combination of thiazole orange and thiazole red as an internal energy transfer-based fluorophore pair in oligonucleotides provides an outstanding analytical tool to follow DNA/RNA hybridization through a distinct fluorescence color change from red to green. Herein, we demonstrate that this concept can be applied to small interfering RNA (siRNA) to monitor RNA integrity in living cells in real ...

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Item type:Article
Date:2013
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich)
Identification Number:
ValueType
10.1021/cb3006616DOI
Dewey Decimal Classification:600 Technology > 615 Pharmacy
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:29275
Owner only: item control page
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