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Metabolic fingerprinting using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry

Almstetter, Martin F. ; Oefner, Peter J. ; Dettmer, Katja



Abstract

Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF-MS) is applied to the comparative metabolic fingerprinting of physiological fluids. Stable isotope-labeled internal standards plus norvaline serve as extraction standards and are added to the blanks, controls and patient samples prior to protein precipitation with methanol. The extracts are evaporated ...

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