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Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography-tandem mass spectrometry

Zhu, Wentao, Stevens, Axel Peter, Dettmer, Katja, Gottfried, Eva, Hoves, Sabine, Kreutz, Marina, Holler, Ernst, Canelas, André B., Kema, Ido and Oefner, Peter J. (2011) Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography-tandem mass spectrometry. Analytical and bioanalytical chemistry 401 (10), pp. 3249-3261.

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Abstract

A sensitive, selective, and comprehensive method for the quantitative determination of tryptophan and 18 of its key metabolites in serum, urine, and cell culture supernatants was developed. The analytes were separated on a C18 silica column by reversed-phase liquid chromatography and detected by electrospray ionization tandem mass spectrometry in positive ion multiple reaction monitoring (MRM) ...

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Item type:Article
Date:December 2011
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie)
Identification Number:
ValueType
21983980PubMed ID
10.1007/s00216-011-5436-yDOI
Classification:
NotationType
Cell LineMESH
Chromatography, High Pressure Liquid/methodsMESH
Culture Media/chemistryMESH
Dendritic Cells/metabolismMESH
HumansMESH
Monocytes/metabolismMESH
Spectrometry, Mass, Electrospray IonizationMESH
Tandem Mass Spectrometry/methodsMESH
Tryptophan/urineMESH
Dewey Decimal Classification:500 Science > 500 Natural sciences & mathematics
500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:30618
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