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Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography-tandem mass spectrometry

Zhu, Wentao ; Stevens, Axel Peter ; Dettmer, Katja ; Gottfried, Eva ; Hoves, Sabine ; Kreutz, Marina ; Holler, Ernst ; Canelas, André B. ; Kema, Ido ; Oefner, Peter J.



Abstract

A sensitive, selective, and comprehensive method for the quantitative determination of tryptophan and 18 of its key metabolites in serum, urine, and cell culture supernatants was developed. The analytes were separated on a C18 silica column by reversed-phase liquid chromatography and detected by electrospray ionization tandem mass spectrometry in positive ion multiple reaction monitoring (MRM) ...

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