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Integrative normalization and comparative analysis for metabolic fingerprinting by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry

Almstetter, Martin F., Appel, Inka, Gruber, Michael, Lottaz, Claudio, Timischl, Birgit, Spang, Rainer, Dettmer, Katja and Oefner, Peter J. (2009) Integrative normalization and comparative analysis for metabolic fingerprinting by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry. Analytical chemistry 81 (14), pp. 5731-5739.

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Abstract

Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC x GC-TOF-MS) was applied to the comparative metabolic fingerprinting of a wild-type versus a double mutant strain of Escherichia coli lacking the transhydrogenases UdhA and PntAB. Using peak lists generated with the Leco ChromaTOF software as input, we developed retention time correction and data alignment tools ...

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Item type:Article
Date:July 2009
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang)
Identification Number:
ValueType
19522528PubMed ID
10.1021/ac900528bDOI
Classification:
NotationType
Automatic Data ProcessingMESH
Chromatography, GasMESH
Escherichia coli/metabolismMESH
Linear ModelsMESH
Mass SpectrometryMESH
Metabolomics/methodsMESH
MutationMESH
NADP/metabolismMESH
NADP Transhydrogenases/metabolismMESH
Principal Component AnalysisMESH
Reproducibility of ResultsMESH
Time FactorsMESH
Dewey Decimal Classification:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:30665
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