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Flow cytometric analysis with a fluorescent formyl peptide receptor ligand as a new method to study the pharmacological profile of the histamine H2-receptor

Werner, Kristin ; Kälble, Solveig ; Wolter, Sabine ; Schneider, Erich H. ; Buschauer, Armin ; Neumann, Detlef ; Seifert, Roland



Abstract

The histamine H2-receptor (H2R) is a Gs protein-coupled receptor. Its activation leads to increases in the second messenger adenosine-3',5'-cyclic monophosphate (cAMP). Presently, several systems are established to characterize the pharmacological profile of the H2R, mostly requiring radioactive material, animal models or human blood cells. This prompted us to establish a flow cytometric analysis ...

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