![[img]](https://epub.uni-regensburg.de/style/images/fileicons/video_x-msvideo.png) | License: Creative Commons Attribution 4.0 Video (AVI) Movie S1 (9MB) | |
| License: Creative Commons Attribution 4.0 Image (TIFF) Figure S1 (1MB) | |
| License: Creative Commons Attribution 4.0 Image (TIFF) Figure S2 (3MB) | |
| License: Creative Commons Attribution 4.0 Image (TIFF) Figure S3 (601kB) |
- URN to cite this document:
- urn:nbn:de:bvb:355-epub-316089
- DOI to cite this document:
- 10.5283/epub.31608
-
Colomb, J., Reiter, L., Blaszkiewicz, J., Wessnitzer, J. and Brembs, Björn (2012) Open Source Tracking and Analysis of Adult Drosophila Locomotion in Buridan’s Paradigm with and without Visual Targets. PLoS ONE 7 (8), e42247.Preview
-
PreviewPreviewColomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Open Source Tracking and Analysis of Adult Drosophila Locomotion in Buridan's Paradigm with and without Visual Targets. [Image] [Currently displayed]Preview
-
Colomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Inexpensive Hardware for Buridan's paradigm. [Image]Preview
-
Colomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Software schematics. [Image]Preview
-
Colomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Calculation of angles and number of walks. [Image]Preview
-
Colomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Activity calculations using different computations give similar results for endogenous locomotion and computer-generated data (grouping codes given below the graph). [Image]Preview
-
Colomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Trajectory parameters of real flies and computer-generated data (grouping codes given below the graph). [Image]Preview
-
Colomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Correlation plot for the different groups of data. [Image]Preview
-
Colomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Transition plots for the different groups of data. [Image]Preview
-
Colomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Fly trajectory metrics for endogenous locomotion (gray bars), Buridan's paradigm with narrow stripes (white bars) or wide stripes (striped pattern). [Image]Preview
-
Colomb, Julien, Reiter, Lutz, Blaszkiewicz, Jedrzej, Wessnitzer, Jan and Brembs, Björn (2012) Reduced state space of fly locomotion behavior after PCA separates the three experimental conditions. [Image]Preview
-
| Item type: | Image | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Journal or Publication Title: | PLoS ONE | ||||||||||
| Publisher: | PubMed Central | ||||||||||
| Volume: | 7 | ||||||||||
| Number of Issue or Book Chapter: | 8 | ||||||||||
| Page Range: | e42247 | ||||||||||
| Date: | 9 August 2012 | ||||||||||
| Institutions: | Biology, Preclinical Medicine > Institut für Zoologie > Neurogenetik (Prof. Dr. Björn Brembs) | ||||||||||
| Identification Number: |
| ||||||||||
| Keywords: | tracking, locomotion, paradigm, targets | ||||||||||
| Dewey Decimal Classification: | 500 Science > 570 Life sciences 500 Science > 590 Zoological sciences | ||||||||||
| Status: | Published | ||||||||||
| Refereed: | Yes, this version has been refereed | ||||||||||
| Created at the University of Regensburg: | No | ||||||||||
| Item ID: | 31608 |
Abstract
Background Insects have been among the most widely used model systems for studying the control of locomotion by nervous systems. In Drosophila, we implemented a simple test for locomotion: in Buridan's paradigm, flies walk back and forth between two inaccessible visual targets [1]. Until today, the lack of easily accessible tools for tracking the fly position and analyzing its trajectory has ...
Abstract
Background
Insects have been among the most widely used model systems for studying the control of locomotion by nervous systems. In Drosophila, we implemented a simple test for locomotion: in Buridan's paradigm, flies walk back and forth between two inaccessible visual targets [1]. Until today, the lack of easily accessible tools for tracking the fly position and analyzing its trajectory has probably contributed to the slow acceptance of Buridan's paradigm.
Methodology/Principal Findings
We present here a package of open source software designed to track a single animal walking in a homogenous environment (Buritrack) and to analyze its trajectory. The Centroid Trajectory Analysis (CeTrAn) software is coded in the open source statistics project R. It extracts eleven metrics and includes correlation analyses and a Principal Components Analysis (PCA). It was designed to be easily customized to personal requirements. In combination with inexpensive hardware, these tools can readily be used for teaching and research purposes. We demonstrate the capabilities of our package by measuring the locomotor behavior of adult Drosophila melanogaster (whose wings were clipped), either in the presence or in the absence of visual targets, and comparing the latter to different computer-generated data. The analysis of the trajectories confirms that flies are centrophobic and shows that inaccessible visual targets can alter the orientation of the flies without changing their overall patterns of activity.
Conclusions/Significance
Using computer generated data, the analysis software was tested, and chance values for some metrics (as well as chance value for their correlation) were set. Our results prompt the hypothesis that fixation behavior is observed only if negative phototaxis can overcome the propensity of the flies to avoid the center of the platform. Together with our companion paper, we provide new tools to promote Open Science as well as the collection and analysis of digital behavioral data.
Movie S1:
Movie of the 3D representation shown in Fig. 9B, see Fig. 9 legend for more information.
Figure S1:
Histogram of speed frequency (on a logarithmic scale, speed is calculated on a sliding window of 1 s) for all 60 flies tested. Bars denote the lower and upper thresholds (1 and 2.7 mm/s, in red and green, respectively).
Figure S2:
Distribution of speed values frequency in the computer-generated data, when no threshold for movement (Tm) is applied. Red bars represent the threshold for movement (8 mm/s = 0.8 mm in 0.1 s) and for jumps (50 mm/s) that delimit the grayed areas of discarded movements.
Figure S3:
Activity in fly endogenous locomotion (gray bars), in Buridan's paradigm with narrow stripes (white bars) or wide stripes (striped pattern). A. The number of pauses is significantly different between the wide stripes situation and the two other situations. B–D. No significant differences in the total activity time (B), the median activity bout duration (C) and the median pause duration (D) in the three situations, although a trend for higher activity can be seen in the wide stripe situation. Bars represent means and error bars standard errors, n = 20 in each group.
Metadata last modified: 29 Sep 2021 07:40
Owner only: item control page
Download Statistics