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Testing Suitability of Cell Cultures for SILAC-Experiments Using SWATH-Mass Spectrometry

Reinders, Y. ; Völler, D. ; Bosserhoff, Anja-Katrin ; Oefner, Peter ; Reinders, Jörg



Abstract

Precise quantification is a major issue in contemporary proteomics. Both stable-isotope-labeling and label-free methods have been established for differential protein quantification and both approaches have different advantages and disadvantages. The present protocol uses the superior precision of label-free SWATH-mass spectrometry to test for suitability of cell lines for a SILAC-labeling ...

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