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Testing Suitability of Cell Cultures for SILAC-Experiments Using SWATH-Mass Spectrometry

Reinders, Y., Völler, D., Bosserhoff, Anja-Katrin, Oefner, Peter and Reinders, Jörg (2016) Testing Suitability of Cell Cultures for SILAC-Experiments Using SWATH-Mass Spectrometry. Methods Mol Biol 1394, pp. 101-108.

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Abstract

Precise quantification is a major issue in contemporary proteomics. Both stable-isotope-labeling and label-free methods have been established for differential protein quantification and both approaches have different advantages and disadvantages. The present protocol uses the superior precision of label-free SWATH-mass spectrometry to test for suitability of cell lines for a SILAC-labeling ...

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Item type:Article
Date:2016
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Medicine > Lehrstuhl für Pathologie
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I
Identification Number:
ValueType
26700044PubMed ID
10.1007/978-1-4939-3341-9_8DOI
Keywords:Label-free proteomics SWATH-MS SILAC Melanoma cells
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Partially
Item ID:33142
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