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Label-free analysis of GPCR-stimulation: The critical impact of cell adhesion

Lieb, Sebastian ; Michaelis, Stefanie ; Plank, Nicole ; Bernhardt, Günther ; Buschauer, Armin ; Wegener, Joachim



Abstract

Label-free cell-based assays have been attracting growing attention in drug research. Optical approaches based on evanescent electric fields (e.g. EPIC, RWG/DMR, SPR) and electrochemical impedance analysis (ECIS, xCELLigence) are by far the most widespread techniques for such purposes. We compared three label-free approaches (ECIS, RWG/DMR and SPR) with respect to the activation of the human ...

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