| Item type: | Article | ||||||
|---|---|---|---|---|---|---|---|
| Journal or Publication Title: | Pharmacological Research | ||||||
| Publisher: | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | ||||||
| Place of Publication: | LONDON | ||||||
| Volume: | 108 | ||||||
| Number of Issue or Book Chapter: | June | ||||||
| Page Range: | pp. 65-74 | ||||||
| Date: | 2016 | ||||||
| Additional Information (public): | Supplementary Material available online. | ||||||
| Institutions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) | ||||||
| Projects (Historical): | GRK 1910, Graduiertenkolleg "Medicinal Chemistry of Selective GPCR Ligands" | ||||||
| Identification Number: |
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| Related URLs: |
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| Keywords: | PROTEIN-COUPLED RECEPTORS; SCAVENGER RECEPTOR; DRUG DISCOVERY; CULTURE; ASSAYS; Label-free; Cell-based assays; G-protein-coupled receptors; Holistic; ECIS; SPR; Dynamic mass redistribution (DMR) | ||||||
| Dewey Decimal Classification: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences | ||||||
| Status: | Published | ||||||
| Refereed: | Yes, this version has been refereed | ||||||
| Created at the University of Regensburg: | Yes | ||||||
| Item ID: | 33747 |
Web of ScienceAbstract
Label-free cell-based assays have been attracting growing attention in drug research. Optical approaches based on evanescent electric fields (e.g. EPIC, RWG/DMR, SPR) and electrochemical impedance analysis (ECIS, xCELLigence) are by far the most widespread techniques for such purposes. We compared three label-free approaches (ECIS, RWG/DMR and SPR) with respect to the activation of the human ...
Abstract
Label-free cell-based assays have been attracting growing attention in drug research. Optical approaches based on evanescent electric fields (e.g. EPIC, RWG/DMR, SPR) and electrochemical impedance analysis (ECIS, xCELLigence) are by far the most widespread techniques for such purposes. We compared three label-free approaches (ECIS, RWG/DMR and SPR) with respect to the activation of the human histamine H-1 receptor (H1R) expressed by U-373 MG glioblastoma and genetically engineered HEK 293T cells. HEK 293T cells were either expressing the hH(1)R alone or in combination with the adhesion protein hMSR1. The beta(2)-adrenergic receptor (beta(2)-AR) expressed by bovine aortic endothelial cells (BAEC) served as a second cell model. Reduced cell adhesion to the surface of the sensing devices affected both, the optical and the impedance-based readout, but became much more obvious in case of RWG- or SPR-based assays. By contrast, the co-expression of hH(1)R and hMSR1 in HEK 293T cells strongly enhanced the signal compared to hH(1)R expression alone. As the sensitivity of the optical readouts is confined to a distance of 100-200 nm from the surface, depending on the wavelength of the incident light, this observation is in accordance with tighter adhesion of the co-transfectants, inducing a shorter distance between the cell membrane and the substrate. Combining ECIS and SPR, allowing for simultaneous registration of both signals for a single cell population, provided a direct correlation of both readouts, when H1R or (beta(2)-AR stimulation was investigated for the same cell populations. Cell adhesion was found to have a critical impact on the results of label-free cell monitoring, in particular when techniques based on evanescent electric fields are applied. (C) 2016 Published by Elsevier Ltd.
Metadata last modified: 25 May 2018 13:56
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