Zusammenfassung
Fluorescence-labeled neuropeptide Y (NPY) has been used in flow cytometric binding assays for the determination of affinity constants of NPY Y-1, Y-2, and Y-5 receptor ligands. Because the binding of fluorescent NPY is insufficient for competition studies at the human Y-4 receptor (hY(4)R), we replaced Glu-4 in hPP with Lys for the derivatization with cyanine-5. Because cy5-[K-4] hPP has high ...
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