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A Simple and Powerful Flow Cytometric Method for the Simultaneous Determination of Multiple Parameters at G-Protein-coupled Receptor Subtypes.

Schneider, Erich ; Mayer, Matthias ; Ziemek, Ralf ; Li, Liantao ; Hutzler, Christoph ; Bernhardt, Günther ; Buschauer, Armin


The quantification of pharmacological parameters at G protein-coupled receptors (GPCRs) is indispensable in drug research but costly and time-consuming when conventional methods are sequentially applied. With neuropeptide Y (NPY) Y(1), Y(2), and Y(5) receptors as model systems, a homogenous flow cytometric method for the simultaneous determination of the affinity, selectivity, and activity of ...


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