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Optimizing the SWATH-MS-workflow for label-free proteomics

Simburger, J. M. ; Dettmer, Katja ; Oefner, Peter J. ; Reinders, Jörg


Quantification of proteins by mass spectrometry at the level of entire proteomes has become a central aspect in contemporary proteomics. Sequential Window Acquisition of all THeoretical fragment-ion spectra (SWATH) mass spectrometry is a label-free protein quantification method enabling parallel quantification of up to several thousand proteins, thereby granting a comprehensive overview of highly ...


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