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Distinct interaction of human and guinea pig histamine H_2-Receptor with guanidine-type agonists,

Kelley, Melissa T. ; Bürckstümmer, Tilmann ; Wenzel-Seifert, Katharina ; Dove, Stefan ; Buschauer, Armin ; Seifert, Roland



Abstract

It is unknown why the potencies and efficacies of long-chained guanidine-type histamine H2-receptor (H2R) agonists are lower at the H2R of human neutrophils than at the H2R of the guinea pig atrium. To elucidate these differences, we analyzed fusion proteins of the human H2R (hH2R) and guinea pig H2R (gpH2R), respectively, and the short splice variant of Gsalpha (Gsalpha S) expressed in Sf9 ...

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