Zusammenfassung
We present a high-performance liquid chromatography (HPLC) and a capillary zone electrophoresis (CZE) method for the chiral separation of 3-phenyl-3-(2-pyridyl)propylamines, -guanidines and -guanidine-N-carboxylic acid esters. The N-alkoxycarbonylguanidines were resolved into enantiomers on a Chiralpak AD column with resolutions ≥2.0. The separation of chloro-substituted ...
Zusammenfassung
We present a high-performance liquid chromatography (HPLC) and a capillary zone electrophoresis (CZE) method for the chiral separation of 3-phenyl-3-(2-pyridyl)propylamines, -guanidines and -guanidine-N-carboxylic acid esters. The N-alkoxycarbonylguanidines were resolved into enantiomers on a Chiralpak AD column with resolutions ≥2.0. The separation of chloro-substituted 3-phenyl-3-(2-pyridyl)propylamines was performed on a Cyclobond I column and a RP18 column, dynamically coated with β-cyclodextrin (CD). The analytical resolution of all compounds was achieved by CZE using α-, β- or γ-CD as chiral selectors. After determination of the best suited CD, buffer pH, CD concentration and capillary temperature were optimized for each individual analyte, resulting in resolutions ≥1.2.
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