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DNA mutation detection using denaturing high-performance liquid chromatography (DHPLC)

Yu, B., Sawyer, N. A., Chiu, C., Oefner, Peter J. and Underhill, P. A. (2006) DNA mutation detection using denaturing high-performance liquid chromatography (DHPLC). Current Protocols in Human Genetics 48 (7), 10.1-7.10.14.

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Other URL: http://onlinelibrary.wiley.com/doi/10.1002/0471142905.hg0710s48/abstract


DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single-base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high-throughput mutation detection and SNP discovery.

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Item type:Article
Date:February 2006
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Identification Number:
18428395PubMed ID
Keywords:denaturing high performance liquid chromatography; mutation detection; DNA heteroduplex; temperature-modulated fractionation; high-throughput
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:No
Item ID:34333
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