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Allelic loss analysis by denaturing high-performance liquid chromatography and electrospray ionization mass spectrometry

Gross, E., Hölzl, Georg, Arnold, N., Hauenstein, E., Jacobsen, A., Schulze, K., Ramser, J., Meindl, A., Kiechle, M. and Oefner, Peter J. (2007) Allelic loss analysis by denaturing high-performance liquid chromatography and electrospray ionization mass spectrometry. Human Mutation 28 (3), pp. 303-311.

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Other URL: http://onlinelibrary.wiley.com/doi/10.1002/humu.20439/abstract


Abstract

Analysis of allelic imbalance is of great importance for understanding tumorigenesis and the clinical management of malignant disease. Fluorescent-based capillary electrophoresis (CE) of highly polymorphic short tandem repeats (STRs) has become the main method used to detect the loss/gain of alleles. However, there is continued interest in the development of techniques that require no ...

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Item type:Article
Date:March 2007
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Identification Number:
ValueType
17109391PubMed ID
10.1002/humu.20439DOI
Keywords:loss of heterozygosity; HPLC; capillary electrophoresis; mass spectrometry; ovarian cancer; PCR fidelity; short tandem repeats
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Partially
Item ID:34381
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