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Allelic loss analysis by denaturing high-performance liquid chromatography and electrospray ionization mass spectrometry

Gross, E. ; Hölzl, Georg ; Arnold, N. ; Hauenstein, E. ; Jacobsen, A. ; Schulze, K. ; Ramser, J. ; Meindl, A. ; Kiechle, M. ; Oefner, Peter J.


Analysis of allelic imbalance is of great importance for understanding tumorigenesis and the clinical management of malignant disease. Fluorescent-based capillary electrophoresis (CE) of highly polymorphic short tandem repeats (STRs) has become the main method used to detect the loss/gain of alleles. However, there is continued interest in the development of techniques that require no ...


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