Hsp90 is a molecular chaperone interacting with hundreds client proteins. Little is known, however, about Hsp90 influence on cancer cell metabolism. Here we show that the inhibition of Hsp90 affects indirectly glycolysis by disrupting mitochondrial insertion of the elements of Translocase of the Outer Membrane (TOM) complex in tumor cells. Improper insertion of Tom40 decreases the abundance of many mitochondrial proteins resulting in reduced mitochondrial activity. This is accompanied by increased production and release of lactate and serine. These results indicate an increased rate of glycolysis with serine synthesis compensating for the loss of energy and mitochondrially derived metabolites, potentially enhancing the metastatic potential of surviving cells. Our results provide novel insights into the effects of Hsp90 inhibition on cancer cell metabolism.