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External calibration with Drosophila whole-cell spike-ins delivers absolute mRNA fold changes from human RNA-Seq and qPCR data

Taruttis, Franziska ; Feist, M. ; Schwarzfischer, Philipp ; Gronwald, Wolfram ; Kube, D. ; Spang, Rainer ; Engelmann, Julia C.



Abstract

Gene expression measurements are typically performed on a fixed-weight aliquot of RNA, which assumes that the total number of transcripts per cell stays nearly constant across all conditions. In cases where this assumption does not hold (e.g., when comparing cell types with different cell sizes) the expression data provide a distorted view of cellular events. Assuming constant numbers of total ...

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