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External calibration with Drosophila whole-cell spike-ins delivers absolute mRNA fold changes from human RNA-Seq and qPCR data

Taruttis, Franziska, Feist, M., Schwarzfischer, Philipp, Gronwald, Wolfram, Kube, D., Spang, Rainer and Engelmann, Julia C. (2017) External calibration with Drosophila whole-cell spike-ins delivers absolute mRNA fold changes from human RNA-Seq and qPCR data. BioTechniques 62 (2), pp. 53-61.

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Other URL: http://www.biotechniques.com/BiotechniquesJournal/2017/February/External-calibration-with-Drosophila-whole-cell-spike-ins-delivers-absolute-mRNA-fold-changes-from-human-RNA-Seq-and-qPCR-data/biotechniques-365611.html


Abstract

Gene expression measurements are typically performed on a fixed-weight aliquot of RNA, which assumes that the total number of transcripts per cell stays nearly constant across all conditions. In cases where this assumption does not hold (e.g., when comparing cell types with different cell sizes) the expression data provide a distorted view of cellular events. Assuming constant numbers of total ...

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Item type:Article
Date:1 February 2017
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang)
Identification Number:
ValueType
28193148PubMed ID
10.2144/000114514DOI
Keywords:C-MYC; RNA sequencing (RNA-Seq); qPCR; gene expression; spike-in; external reference; calibration
Dewey Decimal Classification:500 Science > 500 Natural sciences & mathematics
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Partially
Item ID:35250
Owner only: item control page
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