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Quantification of metabolites by NMR spectroscopy in the presence of protein

Wallmeier, Jens ; Samol, Claudia ; Ellmann, Lisa ; Zacharias, Helena ; Vogl, Franziska C. ; Garcia, Muriel ; Dettmer, Katja ; Oefner, Peter J. ; Gronwald, Wolfram



Zusammenfassung

The high reliability of NMR spectroscopy makes it an ideal tool for large-scale metabolomic studies. However, the complexity of biofluids and, in particular, the presence of macromolecules poses a significant challenge. Ultrafiltration and protein precipitation are established means of deproteinization and recovery of free or total metabolite content, but neither is ever complete. In addition, ...

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