Go to content
UR Home

A split luciferase-based probe for quantitative proximal determination of Gαq signalling in live cells

Littmann, Timo, Ozawa, Takeaki, Hoffmann, Carsten, Buschauer, Armin and Bernhardt, Günther (2018) A split luciferase-based probe for quantitative proximal determination of Gαq signalling in live cells. Scientific Reports 8 (1), p. 17179.

[img]
Preview
License: Creative Commons Attribution 4.0
PDF - Published Version
Download (2MB)
Date of publication of this fulltext: 03 Dec 2018 14:01

at publisher (via DOI)

Other URL: http://doi.org/10.1038/s41598-018-35615-w


Abstract

The earlier an activation of a G protein-dependent signalling cascade at a G protein-coupled receptor (GPCR) is probed, the less amplificatory effects contribute to the measured signal. This is especially useful in case of a precise quantification of agonist efficacies, and is of paramount importance, when determining agonist bias in relation to the β-arrestin pathway. As most canonical assays ...

plus


Export bibliographical data



Item type:Article
Date:2018
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)
Identification Number:
ValueType
10.1038/s41598-018-35615-wDOI
Dewey Decimal Classification:500 Science > 570 Life sciences
500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:38087
Owner only: item control page

Downloads

Downloads per month over past year

  1. Homepage UR

University Library

Publication Server

Contact:

Publishing: oa@ur.de

Dissertations: dissertationen@ur.de

Research data: daten@ur.de

Contact persons