A split luciferase-based probe for quantitative proximal determination of Gαq signalling in live cells

URN to cite this document:: urn:nbn:de:bvb:355-epub-380877
DOI to cite this document:: 10.5283/epub.38087

Littmann, Timo ; Ozawa, Takeaki ; Hoffmann, Carsten ; Buschauer, Armin

; Bernhardt, Günther

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Date of publication of this fulltext: 03 Dec 2018 14:01

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Abstract

The earlier an activation of a G protein-dependent signalling cascade at a G protein-coupled receptor (GPCR) is probed, the less amplificatory effects contribute to the measured signal. This is especially useful in case of a precise quantification of agonist efficacies, and is of paramount importance, when determining agonist bias in relation to the beta-arrestin pathway. As most canonical assays ...