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Independent impedimetric analysis of two cell populations co-cultured on opposite sides of a porous support

Hajek, Kathrin and Wegener, Joachim (2017) Independent impedimetric analysis of two cell populations co-cultured on opposite sides of a porous support. Experimental Cell Research 351 (1), pp. 121-126.

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Other URL: http://doi.org/10.1016/j.yexcr.2017.01.003


Abstract

The transepithelial or -endothelial electrical resistance (TEER) is a very common and routinely recorded parameter describing the expression of barrier-forming cell-cell contacts (tight junctions) in quantitative terms. To determine TEER, barrier-forming cell monolayers are cultured on porous filter supports that separate two fluid compartments. The frequency-dependent impedance of the cell layer ...

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Item type:Article
Date:2017
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener)
Identification Number:
ValueType
10.1016/j.yexcr.2017.01.003DOI
Keywords:CAPILLARY ENDOTHELIAL-CELLS; PLEXUS EPITHELIAL-CELLS; BLOOD-BRAIN-BARRIER; IN-VITRO; TIGHT JUNCTIONS; TRANSPORT; DYNAMICS; CULTURE; MODEL; Impedance analysis; Transepithelial electrical resistance (TEER); Co-cultures; MDCK; Label-free; Cell-based assays; Barrier function
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:38724
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