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A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis

Treiber, Thomas ; Treiber, Nora ; Plessmann, Uwe ; Harlander, Simone ; Daiß, Julia-Lisa ; Eichner, Norbert ; Lehmann, Gerhard ; Schall, Kevin ; Urlaub, Henning ; Meister, Gunter


During microRNA (miRNA) biogenesis, two endonucleolytic reactions convert stem-loop-structured precursors into mature miRNAs. These processing steps can be posttranscriptionally regulated by RNA-binding proteins (RBPs). Here, we have used a proteomics-based pull-down approach to map and characterize the interactome of a multitude of pre-miRNAs. We identify similar to 180 RBPs that interact ...


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