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The Composition of the Arabidopsis RNA Polymerase II Transcript Elongation Complex Reveals the Interplay between Elongation and mRNA Processing Factors

Antosz, Wojciech, Pfab, Alexander, Ehrnsberger, Hans F., Holzinger, Philipp, Köllen, Karin, Mortensen, Simon A. , Bruckmann, Astrid, Schubert, Thomas , Längst, Gernot, Griesenbeck, Joachim , Schubert, Veit , Grasser, Marion and Grasser, Klaus D. (2017) The Composition of the Arabidopsis RNA Polymerase II Transcript Elongation Complex Reveals the Interplay between Elongation and mRNA Processing Factors. The Plant Cell 29 (4), pp. 854-870.

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Other URL: http://doi.org/10.1105/tpc.16.00735


Abstract

Transcript elongation factors (TEFs) are a heterogeneous group of proteins that control the efficiency of transcript elongation of subsets of genes by RNA polymerase II (RNAPII) in the chromatin context. Using reciprocal tagging in combination with affinity purification and mass spectrometry, we demonstrate that in Arabidopsis thaliana, the TEFs SPT4/SPT5, SPT6, FACT, PAF1-C, and TFIIS copurified ...

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Item type:Article
Date:2017
Institutions:Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck
Identification Number:
ValueType
10.1105/tpc.16.00735DOI
Keywords:DIRECTED DNA METHYLATION; LOCUS-C CHROMATIN; PAF1 COMPLEX; P-TEFB; GENE-EXPRESSION; SACCHAROMYCES-CEREVISIAE; AFFINITY PURIFICATION; PLANT-GROWTH; SH2 DOMAIN; PROTEIN;
Dewey Decimal Classification:500 Science > 570 Life sciences
500 Science > 580 Botanical sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:39118
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