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Solid-State NMR H-N-(C)-H and H-N-C-C 3D/4D Correlation Experiments for Resonance Assignment of Large Proteins

Fraga, Hugo ; Arnaud, Charles-Adrien ; Gauto, Diego F. ; Audin, Maxime ; Kurauskas, Vilius ; Macek, Pavel ; Krichel, Carsten ; Guan, Jia-Ying ; Boisbouvier, Jerome ; Sprangers, Remco ; Breyton, Cécile ; Schanda, Paul



Abstract

Solid-state NMR spectroscopy can provide insight into protein structure and dynamics at the atomic level without inherent protein size limitations. However, a major hurdle to studying large proteins by solid-state NMR spectroscopy is related to spectral complexity and resonance overlap, which increase with molecular weight and severely hamper the assignment process. Here the use of two sets of ...

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