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Library Selection with a Randomized Repertoire of (βα)8-Barrel Enzymes Results in Unexpected Induction of Gene Expression

Rohweder, Bettina ; Lehmann, Gerhard ; Eichner, Norbert ; Polen, T. ; Rajendran, Chitra ; Ruperti, Fabian ; Linde, Mona ; Treiber, Thomas ; Jung, Oona ; Dettmer, Katja ; Meister, Gunter ; Bott, M. ; Gronwald, Wolfram ; Sterner, Reinhard



Abstract

The potential of the frequently encountered (βα)8-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection in vivo. For this purpose, the genes encoding 52 different phosphate-binding (βα)8-barrel proteins were subjected to error-prone PCR and cloned into an expression plasmid. The resulting mixed repertoire was used to transform different ...

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