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Library Selection with a Randomized Repertoire of (βα)8-Barrel Enzymes Results in Unexpected Induction of Gene Expression

Rohweder, Bettina, Lehmann, Gerhard, Eichner, Norbert, Polen, T., Rajendran, Chitra, Ruperti, Fabian, Linde, Mona, Treiber, Thomas, Jung, Oona, Dettmer, Katja , Meister, Gunter, Bott, M., Gronwald, Wolfram and Sterner, Reinhard (2019) Library Selection with a Randomized Repertoire of (βα)8-Barrel Enzymes Results in Unexpected Induction of Gene Expression. Biochemistry 58 (41), pp. 4207-4217.

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Other URL: https://pubs.acs.org/doi/10.1021/acs.biochem.9b00579


Abstract

The potential of the frequently encountered (βα)8-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection in vivo. For this purpose, the genes encoding 52 different phosphate-binding (βα)8-barrel proteins were subjected to error-prone PCR and cloned into an expression plasmid. The resulting mixed repertoire was used to transform different ...

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Item type:Article
Date:15 October 2019
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie
Identification Number:
ValueType
31557000PubMed ID
10.1021/acs.biochem.9b00579DOI
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Partially
Item ID:40835
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