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Robust Metabolite Quantification from J-Compensated 2D 1H-13C-HSQC Experiments

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Weitzel, Alexander ; Samol, Claudia ; Oefner, Peter J. ; Gronwald, Wolfram
License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 13 Nov 2020 05:50


The spectral resolution of 2D H-1-C-13 heteronuclear single quantum coherence (H-1-C-13-HSQC) nuclear magnetic resonance (NMR) spectra facilitates both metabolite identification and quantification in nuclear magnetic resonance-based metabolomics. However, quantification is complicated by variations in magnetization transfer, which among others originate mainly from scalar coupling differences. ...


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